Biomolecular Integrations, Inc. offers ELISA screening of antibody titers by coating free peptides or peptides coupled to a carrier protein other than used for immunization using our optimized ELISA protocols. This way, antibodies to carrier proteins are not detected. We perform ELISA on individual bleeds along with pre-immune serum at 5 dilutions (1:10 , 1:100, 1:1K, 1:10 K and 1:100K). The ELISA results are tabulated in an easy to understand format. Phospho-peptide antibodies are characterized by ELISA using phospho and dephospho peptides also at 5 dilutions. The Phospho-specific antibodies are later isolated by sequential affinity purification on dephospho and phospho peptide affinity matrices. The detailed methodology for ELISA is provided with each antibody progress report for easy reproduction of results in investigators laboratory if needed. All ELISA reagents are also available from Biomolecular Integrations, Inc. We also characterize antibodies by Western Blot analysis.