Day 0

Pre-immune bleed collected and stored at -80 ⁰Cuntil the Day 63 bleed.
Immunize with 100-200ug protein in Complete Freund's Adjuvant (CFA). Acrylamide protein extracts can only be injected subcutaneously.

Day 28

First booster immunization with 50-100ug protein in Incomplete Freund's Adjuvant (IFA)

Day 42

Second booster injection with 50-100ug protein in IFA.

Day 56

Test bleed. Thirst booster injection with 25ug protein in IFA. Inject 25ug protein intravenously (antigen must be soluble in PBS or water) using no adjuvant. If this is not available, we will skip the intravenous injection.

Day 63

Production bleed. We will ship pre-immune bleed, the Day 56 test bleed and this production bleed. The investigator tests the sera, determines titer and the quality of antibodies, and informs us if for any changes in the protocol. We also offer antibody titration service by ELISA.

Day 70

Fourth booster injection with 25-50ug protin in IFA.

Day 84

Test bleed. We will do these bleeds every 14 days unless the investigator specifies.

After Day 84

See Continuation of your Protocol below.