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Mouse Monoclonal Antibody Production Service

Biomolecular Integrations provides comprehensive monoclonal antibody production
service.using our state of-the-art facilities and experienced team of well rounded
professionals. We also offer large scale ascites production and antibody purification and
characterization services.
We can use antigens (proteins, peptides or recombinant proteins) provided by customers
or produced in house by Biomolecular Integrations to immunize mouse. We generally
follow our standardized protocol for the fusion of myeloma cells and antibody-producing
B-lymphocytes, and clonal screening and expansion. However, we can also customize the
protocol according to customer’s needs. We deliver monoclonal antibody projects in the
most cost-effective and timely manner.
●Antigens (recombinant proteins, peptides, or others) provided by customers or
produced at Biomolecular Integrations.
●Biomolecular Integrations will fuse SP2/0 with 1 mouse spleen cells
selected from 5 immunized BALB/c mice with the best ELISA titer.
Customers may also request customized package. We will work with you to
come up with a package that is the most cost effective and applicable to your
needs.
●Monoclonal antibodies are typically produced in 4-6 months.
Validation of Non-characterized Antibody: We also provide additional services for
characterization of antibodies on for ELISA assays, immunoprecipitation, Western
blotting, and Immunohistochemistry using normal and disease tissues.
Our Guarantees: We guarantee two positive clones for monoclonal antibody
development if peptide antigens were designed, synthesized, and conjugated by
Biomolecular Integrations. ELISA titers are guaranteed to reach >25,000.

Biomolecular Integrations provides comprehensive monoclonal antibody productionservice.using our state of-the-art facilities and experienced team of well roundedprofessionals. We also offer large scale ascites production and antibody purification andcharacterization services.

 
We can use antigens (proteins, peptides or recombinant proteins) provided by customersor produced in house by Biomolecular Integrations to immunize mouse. We generallyfollow our standardized protocol for the fusion of myeloma cells and antibody-producingB-lymphocytes, and clonal screening and expansion. However, we can also customize theprotocol according to customer’s needs. We deliver monoclonal antibody projects in themost cost-effective and timely manner.

 
●Antigens (recombinant proteins, peptides, or others) provided by customers orproduced at Biomolecular Integrations.

 
●Biomolecular Integrations will fuse SP2/0 with 1 mouse spleen cellsselected from 5 immunized BALB/c mice with the best ELISA titer. Customers may also request customized package. We will work with you tocome up with a package that is the most cost effective and applicable to yourneeds.

 

●Monoclonal antibodies are typically produced in 4-6 months.


Validation of Non-characterized Antibody: We also provide additional services forcharacterization of antibodies on for ELISA assays, immunoprecipitation, Westernblotting, and Immunohistochemistry using normal and disease tissues.

 
Our Guarantees: We guarantee two positive clones for monoclonal antibodydevelopment if peptide antigens were designed, synthesized, and conjugated byBiomolecular Integrations. ELISA titers are guaranteed to reach >25,000.

 
Monoclonal antibody production service is divided into different phases. The customerhas the option to be invoiced for each phase at its initiation.
Catalog.Number

Phase

 ...Price....
B1S201 Phase I: Immunization（5 mice/antigen）& Sera Testing by ELISA

$500
B1S202 Phase II: Fusion and Colony Screening

$1800
B1S203 Phase III: Subcloning, Colony Expansion & Frozen

$500
B1S204 Phase IV: Isotyping and Ascites Production (2 clones)

$500
B1S205 Total Price (Phase I – IV)

$3300
B1S206 Phase V: Antibody Purification from Ascites by Protein A/G (2 clones, 3mg each)

$500
B1S207 Total Price (Phase I – V)

$3800
B1S208 Charge for each additional clone (phase IV, V)

$400
BIS105 The customer can provide a ready-to-inject antigen When using a peptide supplied by the customer, additional charge for conjugation to career protein is $199

Customer may request one of product formats for final delivery:

- 2 mouse hybridomas

- 2 mouse hybridoma plus 2-5mg mouse IgGs from Protein A/G purified ascites

- 2 paired mouse hybridomas plus 2-5mg of mouse IgGs for sandwich ELISA (for protein antigens only)

2 mouse hybridomas 2 mouse hybridomas and 2-5mg of Protein A/G purified mouse IgGs 2 Hybridomas and purified IgGs paired for Sandwich ELISA
Antigen Format Quantitiy Antigen Quality Catalog# BIS210 Catalog# BIS2011 Catalog# BIS212
Peptide antigens from customers ~ 5 mg >85% Pure by HPLC Correct MW by MS $3300               (17-19 weeks) $3800 (20-22 weeks) $7600 (for proteins-antigens only) (28-30 weeks)
Protein antigens from customers ~ 5 mg > 90% Pure and correct MW by SDS-PAGE $3300               (17-19 weeks) $3800 (20-22 weeks) $7600 (for proteins-antigens only) (28-30 weeks)
Peptide antigens from customers ~ 10 mg >85% Pure by HPLC Correct MW by MS $3600               (19-21 weeks $4100 (22-24 weeks)
Protein sequences(Peptide design and synthesis at Biomolecular Integrations) ~ 10 mg >85% Pure by HPLC Correct MW by MS $3600               (19-21 weeks) $4100 (22-24 weeks)
cDNA clones (protein production at Biomolecular Integrations) ~ 10 mg 90% Pure and correct MW by SDS-PAGE $4300               (22-24 weeks) $4800 (25-27 weeks) $9600 (33-35 weeks)