Wednesday, February 22, 2012
   
Text Size
contactus

 

Subcellular Fractions

Eukaryotic cells perform essential cellular functions in specialized membrane bound subcellular compartments or organelles. These subcellular or intracellular compartments exist as isolated discrete bodies of various shapes and size that perform specialized cellular function. Understanding their biogenesis and maintenance of their integrity in performing specialized functions is a fundamental challenge in cell biology. Often times, it is utmost important to obtain isolated preparations of these organelles to study their biochemical composition, structure and function under varying physiological or disease states. Protein and lipid composition as well as shape and size of various organelles differ from each other. This has enabled scientists to isolate them as enriched subcellular fractions.

Biomolecular Integrations has developed effective methods to isolate various subcellular fractions with minimized cross contamination and enriched organelle specific biomarkers. These preparations are suitable for use in basic cell biology as well as in drug discovery research. We have also developed buffers and reagents that allow extraction of proteins from specific organelles such as membranes, cytoplasm, nucleus, mitochondria and microsomes, Because of enriched protein contents, these extracts offer useful tools for rapidly growing protomics research particularly allowing analysis of otherwise low abundant proteins.

Scientists at Biomolecular Integrations have over 25 years of experience in subcellular fractionation techniques. Please check the availability of ready made subcellular fractions as frozen stocks.

Additionally, we provide comprehensive service and customized isolation of subcellular fractionations from a number of tissues and species as required for your research. All subcellular fractions are characterized for enrichment of biochemical markers and shipped as frozen stocks on dry ice that can be stored at -70 0C.

Fractions: cytosols, microsomes, nucleus, mitochondria, Golgi bodies, lysosomes, peroxisomes, endosomes etc.

Tissues: liver, kidney, lung, heart intestine, testis, pancreas, brain etc.

Species: human, mouse, rats, rabbits, monkeys etc.

We also offer subcellular fractionation from any cell line that grow in suspension or attach to culture dishes. Our customized subcelllular fractionation service will meet your requirement yet offering economical way to your research.


Subcellular Fraction and Organelle Specific Protein Extracts
While offering buffers and reagents for isolation of enriched subcellular fractions and organelles such as nucleus, mitochondia, microsomes, lysosomes and peroxisomes, we also offer ready-made and customized subcellular fraction specific protein extracts from these organelles.

Whole Cell and Tissue Extracts

We also carry whole cell lysates from a wide range cell lines to use as positive controls in your Western blotting experiments. These cell lysates are mixed with SDS-PAGE loading buffer (Laemmli buffer) ready to use on SDS gels. Cell lysates are also available in native lysis buffer most suitable for immuniprecipitation or ELISA applications.

We also carry various organ specific tissue extracts such as from liver, kidney, lung, heart intestine, testis, pancreas, brain etc. from rat, mouse, rabbit, human and other species. 


Isolation of Primary Cells

We provide comprehensive services and customized isolation of primary cells from tissues of your choice. Our isolated hepatocytes are most suitable for drug screening and cytotoxicity studies.

 

For more information and availability

Please contact us

1-501-661-0202 or This e-mail address is being protected from spambots. You need JavaScript enabled to view it